Adipocyte morphology and leptin signaling in rat offspring from mothers supplemented with flaxseed during lactation

详细信息    查看全文

• 作者：Mariana Sarto Figueiredo ; Ph.D.^a ; Magna Cottini da Fonseca Passos ; Ph.D.^a ; Isis Hara Trevenzoli ; Ph.D.^b ; Aline Andrade Troina ; Ph.D.^a ; Aluana Santana Carlos ; M.S.^c ; Celly Cristina Alves Nascimento-Saba ; Ph.D.^c ; Mabel Carneiro Fraga ; Ph.D.^a ; ^d ; Alex C. Manhã ; es ; Ph.D.^d ; Elaine de Oliveira ; Ph.D.^a ; Patrí ; cia Cristina Lisboa ; Ph.D.^a ; Egberto Gaspar de Moura ; Ph.D.^a ; ^{egbertomoura@globo.com}
• 关键词：Adipose tissue ; Flaxseed ; Lactation ; Leptin signaling and programming
• 刊名：Nutrition
• 出版年：2012
• 出版时间：March 2012
• 年：2012
• 卷：28
• 期：3
• 页码：307-315
• 全文大小：722 K

文摘

Objective

We have recently shown that maternal flaxseed supplementation during lactation induces insulin resistance in adult offspring. Here, we studied the effects of maternal dietary flaxseed during lactation on adipocyte morphology and leptin signaling in the hypothalamic-pituitary-thyroid axis as well as on behavioral traits in the adult progeny.

Methods

Lactating rats were fed a control (C) diet or a diet with 25 % flaxseed (F). After weaning, pups received a standard diet until postnatal day (PN) 180. Male offspring were killed at PN21 and 180. Data were considered significant at P < 0.05.

Results

Weaned F rats presented a lower total and subcutaneous fat mass and higher subcutaneous adipocyte area (+48 % ), but at adulthood they presented higher subcutaneous and visceral adipocyte areas (+40 % and 1.9-fold increase, respectively), with no change in body fat mass. At PN21, F pups had hyperleptinemia (+69 % ), lower T₃ (?3 % ), higher TSH (2.1-fold increase), higher pituitary leptin receptor (Ob-R, +11 % ), signal transducer and activator of transcription 3 (STAT3, +21 % ), and phosphorylated-STAT3 (p-STAT3, +77 % ) protein content. Adult F offspring only showed lower T₄ (?8 % ) and higher thyroid Ob-R (+52 % ) expression. Maternal flaxseed intake during lactation did not result in behavioral changes in the adult offspring.

Conclusions

Maternal flaxseed supplementation decreases offspring adiposity and increases pituitary leptin signaling at weaning, but it induces hypertrophic adipocytes and higher thyroid leptin receptor in adulthood. The present data suggest that extensive use of flaxseed during lactation is undesirable.