Detection of alloantibody-mediated complement activation: A diagnostic advance in monitoring kidney transplant rejection?
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- 作者：Georg A. Bö ; hmiga ; georg.boehmig@meduniwien.ac.at" class="auth_mail" title="E-mail the corresponding author ; Zeljko Kikica ; Markus Wahrmanna ; Farsad Eskandarya ; Arezu Z. Aliabadib ; Gerhard J. Zlabingerc ; Heinz Regeled ; Helmut E. Feuchte
- 关键词：ABMR ; antibody-mediated rejection ; CDC ; complement-dependent cytotoxicity ; CDCXM ; complement-dependent cytotoxicity crossmatch ; DSA ; donor-specific antibodies ; DTT ; dithiothreitol ; EDTA ; ethylenediaminetetraacetic acid ; FCXM ; flow cytometry crossmatch ; HLA ; human leukocyte antigen ; LCL ; lymphoblastoid cell lines ; MFI ; mean fluorescence intensity ; PTC ; peritubular capillaries ; SAB ; single antigen bead ; XM ; crossmatch
- 刊名：Clinical Biochemistry
- 出版年：2016
- 出版时间：March 2016
- 年：2016
- 卷：49
- 期：4-5
- 页码：394-403
- 全文大小：970 K
文摘
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We review the role of classical complement activation in kidney transplantation.
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We discuss the role of C4d staining as a marker of antibody-mediated rejection.
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Bead arrays enable in vitro detection of alloantibody-triggered complement deposition.
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In vitro complement binding assays may help uncover harmful HLA alloreactivity.