Quantification of PEGylated proteases with varying degree of conjugation in mixtures: An analytical protocol combining protein precipitation and capillary gel electrophoresis
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- 作者：Josefine Morgenstern Josefine.Morgenstern@kit.edu" class="auth_mail" title="E-mail the corresponding author ; Markus Busch ; Pascal Baumann Pascal.Baumann@kit.edu" class="auth_mail" title="E-mail the corresponding author ; Jü ; rgen Hubbuch ; juergen.hubbuch@kit.edu" class="auth_mail" title="E-mail the corresponding author ;
- 关键词：Autocatalysis ; Protease PEGylation ; TCA precipitation ; PEGamer quantification ; High-throughput capillary gel electrophoresis
- 刊名：Journal of Chromatography A
- 出版年：2016
- 出版时间：2 September 2016
- 年：2016
- 卷：1462
- 期：Complete
- 页码：153-164
- 全文大小：3669 K
文摘
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High throughput analytic approach for the quantification of PEGylated proteases.
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Capillary electrophoresis as an alternative to chromatography for the analysis of PEGylated proteins.
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Suppression of autocatalytic proteolysis by protein precipitation as sample preparation step.
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Peak base line separation of individual PEGylated species.