Performance evaluation of commercial ELISA kits for screening of furazolidone and furaltadone residues in fish

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• 作者：Edward L.E. Jester ; Ann Abraham ; Yuesong Wang ; Kathleen R. El Said ; Steven M. Plakas
• 关键词：ELISA ; Furazolidone ; Furaltadone
• 刊名：Food Chemistry
• 出版年：2014
• 出版时间：15 February, 2014
• 年：2014
• 卷：145
• 期：Complete
• 页码：593-598
• 全文大小：306 K

文摘

Regulatory monitoring for nitrofuran drug residues in aquaculture products has largely focused on LC-MS/MS. In addition, there is a need for facile and high-throughput screening methods for monitoring programs. We evaluated the performance of Ridascreen (R-Biopharm) ELISA kits for nitrofuran drug residues in fish muscle, with verification by LC-MS/MS. Kits were available for 3-amino-2-oxazolidinone (AOZ) and 3-amino-5-morpholino-methyl-2-oxazolidinone (AMOZ) side-chains of furazolidone and furaltadone, respectively. We found good repeatability in fortified and incurred muscle samples, with RSDs ranging from 1.8 % to 7.6 % . Recoveries of AOZ and AMOZ from muscle fortified at levels of 0.5-2 ng/g ranged from 98 % to 114 % . Excellent selectivity was demonstrated. The minimum detection limits (MDLs) for AOZ and AMOZ in muscle were 0.05 and 0.2 ng/g, respectively. ELISA data were highly correlated with those of LC-MS/MS. Results of this study support the use of these kits as screening assays for nitrofuran residues in fish muscle.