Amphiphilic and biodegradable methoxy polyethylene glycol-block-(polycaprolactone-graft-poly(2-(dimethylamino)ethyl methacrylate)) as an effective gene carrier

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• 作者：Shutao Guo ; Yuanyu Huang ; Tuo Wei ; Wendi Zhang ; Weiwei Wang ; Daoshu Lin ; Xu Zhang ; Anil Kumar ; Quan Du ; Jinfeng Xing ; Li ; ong Deng ; Zicai Liang ; Paul C. Wang ; Anjie Dong ; Xing-Jie Liang
• 关键词：Poly( ; //www.sciencedirect.com/scidirimg/entities/25b.gif"" alt=""var epsilon"" title=""var epsilon"" border=""0"">-caprolactone) ; Gene therapy ; Degradable polymers ; Poly(2-(dimethylamino ethyl)methacrylate) ; Amphiphilic copolymers
• 刊名：Biomaterials
• 出版年：2011
• 出版时间：January 2011
• 年：2011
• 卷：32
• 期：3
• 页码：879-889
• 全文大小：2134 K

文摘

A group of amphiphilic cationic polymers, methoxy polyethylene glycol-block-(polycaprolactone-graft-poly(2-(dimethylamino)ethyl methacrylate)) (PECD), were synthesized by combining ring-opening polymerization (ROP) and atom transfer radical polymerization (ATRP) methods to form nanoparticles (NPs). The structures of these amphiphilic cationic polymers were characterized by ¹H NMR measurement. The PECD NPs have hydrophobic cores covered with hydrophilic PEG and cationic PDMAEMA chains. These self-assembly nanoparticles were characterized by dynamic light scattering (DLS) technique. PECD NPs can effectively condense DNA to form compact complexes of the size 65–160 nm suitable for gene delivery. The in vitro gene transfection studies of HeLa and HepG2 cells show that PECD NPs have better transfection efficiency compared to polyethylenimine (PEI) and Lipofectamine 2000 at low dose (N/P = 5). The cytotoxicity result shows that PECD NPs/DNA complexes at the optimal N/P ratio for transfection have comparable toxicity with PEI and Lipofectamine. These results indicate that PECD NPs have a great potential to be used as efficient polymeric carriers for gene transfection.