Rapid detection of Salmonella in raw chicken breast using real-time PCR combined with immunomagnetic separation and whole genome amplification

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• 作者：Ji-Yeon Hyeon ; Xiangyu Deng ; ^{xdeng@uga.edu}
• 关键词：Salmonella ; Detection ; Chicken ; Whole genome amplification ; IMS ; MDA ; Real-time PCR
• 刊名：Food Microbiology
• 出版年：2017
• 出版时间：May 2017
• 年：2017
• 卷：63
• 期：Complete
• 页码：111-116
• 全文大小：774 K
• 卷排序：63

文摘

Rapid detection of Salmonella from raw chicken breast with no or shortened culture enrichment. High sensitivity with LOD at ∼10 CFU/g without culture enrichment and ∼0.1 CFU/g with a 4 h culture enrichment. Affordable assays by adding less than $10/sample to conventional real-time PCR.