Evaluation experiments demonstrate that the HID-Ion AmpliSeq™ Identity Panel is a well-performed, robust, reliable and high informative NGS-SNP assay.

Some underperformed loci were identified including strand bias, low locus coverage, heterozygote imbalance and high background signals.

Full profile could be obtained from as low as 100 pg input DNA. Compared with STR assays, this panel gives prominence to advantage on detection to degraded sample.

F_ST across all 90 autosomal SNP loci showed no significant difference between Southern and Northern Chinese Han or between male and female samples.

Forensic parameters for A-SNPs were calculated as >99.999% (CDP), 0.999999724 (CPE), 1.390 × 10¹¹ (CLR of trios) and 2.361 × 10⁶ (CLR of duos). HD for Y-SNPs was equal to 0.684.