Saponin determination, expression analysis and functional characterization of saponin biosynthetic genes in Chenopodium quinoa leaves
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- 作者：Jennifer Fiallos-Juradoa ; b ; Jacob Pollierc ; d ; Tessa Mosesc ; d ; Philipp Arendtc ; d ; g ; h ; Noelia Barriga-Medinaa ; Eduardo Morilloi ; Venancio Arahanab ; Maria de Lourdes Torresb ; Alain Goossensc ; d ; Antonio Leon-Reyesa ; aleon@usfq.edu.ec" class="auth_mail" title="E-mail the corresponding author
- 关键词：βAS ; β-amyrin synthase ; AOS ; Allene Oxide Synthase ; CqbAS1 ; Chenopodium quinoa β- amyrin synthase ; OSC ; 2 ; 3-oxidosqualene cyclase ; MON1 ; Monensin Sensitivity 1 ; GC&ndash ; MS ; Gas Chromatography&ndash ; Mass spectrometry ; qPCR ; Quatitative Real-Time PCR ; RNA-seq ; RNA-Sequencing ; MeJA ; Methyl Jasmonate
- 刊名：Plant Science
- 出版年：2016
- 出版时间：September 2016
- 年：2016
- 卷：250
- 期：Complete
- 页码：188-197
- 全文大小：2294 K
文摘
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We quantified saponin content in quinoa leaves treated with methyl jasmonate.
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We found that methyl jasmonate induces accumulation of saponins in quinoa leaves and induces the expression of saponin biosynthetic genes in quinoa.
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Reference genes for qPCR analysis were identified by bioinformatics analysis of available RNA-Seq data.
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We cloned three genes involved in saponin biosynthesis.
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We characterized these genes by functional analyses in yeast.