Emerging food allergens: Identification of polyphenol oxidase as an important allergen in eggplant (Solanum melongena L.)
详细信息 查看全文
- 作者：Bheemanapalli N. Harish Babu<sup>1sup> ; Anthony Wilfred ; Yeldur P. Venkatesh<sup> ; sup><sup>venkatyp@yahoo.comsup>
- 关键词：ARP ; allergen representative peptide ; 4-MC ; 4-methyl catechol ; PPO ; polyphenol oxidase ; PS ; phenyl-Sepharose ; SmePPO4 ; Solanum melongena PPO4 ; SPT ; skin prick test
- 刊名：Immunobiology
- 出版年：2017
- 出版时间：February 2017
- 年：2017
- 卷：222
- 期：2
- 页码：155-163
- 全文大小：1568 K
- 卷排序：222
文摘
Six cases of food allergy to eggplant (Solanum melongena L.) were investigated. Skin prick test (SPT), allergen-specific IgE to eggplant were positive in all cases. Eggplant peel concentrate fractionated on phenyl-Sepharose into PS1-PS5 pools. PS2 pool found to be high in specific activity for polyphenol oxidase (PPO). 64 kDa protein in PS2 identified as an allergen (SmePPO4) by immunoblot/bioinformatics.