Germ cell responses to doxorubicin exposure in vitro

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• 作者：Khaled Habas ; Diana Anderson ; Martin. H. Brinkworth ; ^{m.h.brinkworth@bradford.ac.uk}
• 关键词：DNA damage ; Apoptosis ; Doxorubicin ; Comet assay ; TUNEL assay ; Male germ cells
• 刊名：Toxicology Letters
• 出版年：2017
• 出版时间：4 January 2017
• 年：2017
• 卷：265
• 期：Complete
• 页码：70-76
• 全文大小：1089 K
• 卷排序：265

文摘

Purified cells were treated with different concentrations of the genotoxic agent Doxorubicin (Dox) and assessed for DNA damage and apoptosis. Exposure of the purified germ cells to Dox yielded significant increases in DNA damage and apoptosis. Dox disrupts spermatogenesis by causing DNA damage and apoptosis in spermatogonia, spermatocytes and spermatids. The effects of Dox were cell type- and exposure-dependent with the strongest responses at the highest concentration in spermatogonia and a lack of response in spermatids at the lowest concentration.