Evaluation of neurobehavioral and neuroinflammatory end-points in the post-exposure period in rats sub-acutely exposed to manganese

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• 作者：Santos Dinamene ; Batoreu M. Camila ; I. Tavares de Almeida ; L. Davis R ; all ; Mateus M. Luisa ; Andrade V ; a ; Ramos Ruben ; Torres Edite ; Aschner Michael ; A.P. Marreilha dos Santos
• 关键词：Manganese neurotoxicity ; Neurobehavioral assays ; TNF-伪 ; GFAP ; Neuroinflammation
• 刊名：Toxicology
• 出版年：6 December, 2013
• 年：2013
• 卷：314
• 期：1
• 页码：95-99
• 全文大小：737 K

文摘

Manganese (Mn) can cause manganism, a neurological disorder similar to Parkinson鈥?Disease (PD). The neurobehavioral and neuroinflammatory end-points in the Mn post exposure period have not been studied yet. Rats were injected on alternate days with 8 doses of MnCl₂ (25 mg/kg) or saline, then euthanized 1, 10, 30 or 70 days following the last dose. Whole-blood (WB) (p < 0.05), urine (p < 0.05) and brain cortical (p < 0.0001) Mn levels were significantly increased 24 h after the last dose. Decreases in the rats鈥?ambulation were noted 1, 10 and 30 days after the last Mn dose (p < 0.001; p < 0.05; p < 0.001, respectively) and also in the rearing activity at the four time-points (p < 0.05). Cortical glial fibrillary acid protein immunoreactivity (GFAP-ir) was significantly increased at 1, 10, 30 (p < 0.0001) and 70 (p < 0.001) days after the last Mn dose, as well as tumor necrosis 伪 (TNF-伪) levels (p < 0.05) but just on day 1. Taken together, the results show that, during the 70-day clearance phase of Mn, the recovery is not immediate as behavioral alterations and neuroinflammation persist long after Mn is cleared from the cortical brain compartment.