A technique for determining Young's modulus of biological objects using atomic-force microscopy in the wide temperature range below Rt

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• 作者：Kseniya I. Krymskaya ; ^{ksenia.krymskaya@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Nataliya V. Andreeva ^{nvandr@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Aleksey V. Filimonov ^{filimonov@rphf.spbstu.ru" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Cryogenic atomic-force microscopy ; Force-distance curve ; Young's modulus
• 刊名：St. Petersburg Polytechnical University Journal: Physics and Mathematics
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：2
• 期：3
• 页码：217-223
• 全文大小：1094 K

文摘

In the paper, we suggest widening the scope of cryogenic atomic-force microscopy in order to determine Young's modulus of biological objects over a temperature range stretching from 30 to 300 K. A new technique assumes some modification of the arrangement for the optical system recording the probe position in the cryogenic atomic-force microscope and making measurements of approach and retraction force-distance curves under these conditions. Young's modulus is determined from the obtained data by calculation taking into account the temperature dependence of a cantilever spring constant. As an example we have obtained a temperature dependence of Young's modulus for polylysine in the temperature range stretching from 60 to 300 K (RT) using the proposed technique.