Synthesis of glycinamides using protease immobilized magnetic nanoparticles

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• 作者：Abha Sahu ; Pallavi Sharad Badhe ; Ravindra Adivarekar ; Mayur Ramrao Ladole ; Aniruddha Bhalchandra Pandit ; ^{ab.pandit@ictmumbai.edu.in}
• 关键词：Protease ; Magnetic nanoparticles ; Immobilization ; Glycinamides ; Response surface methodology
• 刊名：Biotechnology Reports
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：12
• 期：Complete
• 页码：13-25
• 全文大小：4762 K
• 卷排序：12

文摘

Protease producing Bacillus subtilis was isolated from animal slaughter house waste. Protease was covalently immobilized on amino-functionalized magnetic nanoparticles (AMNPs). To our knowledge, for the first time these magnetic nano biocatalyst was used for the synthesis of series of novel glycinamides. Parameters for glycinamides synthesis such as, pH, temperature and time were optimized using Response Surface Methodology. Reusability study showed that protease immobilized MNPs retain up to 70% of initial activity after 8th cycles of reuse for the synthesis.