Hyperbaric oxygen preconditioning inhibits skin flap apoptosis in a rat ischemia-reperfusion model

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• 作者：Yi-Ding Xiao ; MD^a ; ¹ ; Yun-Qi Liu ; MS^b ; ¹ ; Jia-La Li ; MS^b ; Xue-Mei Ma ; PhD^b ; ^{xmma@bjut.edu.cn" class="auth_mail" title="E-mail the corresponding author} ; You-Bin Wang ; MD^a ; ^{wybenz@sina.com" class="auth_mail" title="E-mail the corresponding author} ; Yi-Fang Liu ; MS^b ; Ming-Zi Zhang ; MS^a ; Peng-Xiang Zhao ; PhD^b ; Fei Xie ; PhD^b ; Zi-Xuan Deng ; BS^b
• 关键词：Hyperbaric oxygen ; Ischemia&ndash ; reperfusion ; Skin flaps ; Apoptosis
• 刊名：Journal of Surgical Research
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：199
• 期：2
• 页码：732-739
• 全文大小：1807 K

文摘

Hyperbaric oxygen (HBO) improves skin flap function and inhibits partial necrosis induced by ischemia–reperfusion (I/R) injury. Our study aimed to evaluate the mechanism underlying HBO regulation of the antiapoptosis factors associated with I/R injury of skin flaps.

Methods

The rats were divided into sham surgery, I/R, and HBO groups. Rats from the HBO group received HBO preconditioning followed by I/R surgery. Blood perfusion of the skin flaps was measured with laser Doppler flowmeters. Tissue morphology and apoptosis were subsequently assessed based on hematoxylin-eosinhe and terminal deoxynucleotidyl transferase dUTP nick-end labeling staining. Protein expression of phosphorylated apoptosis signal-regulating kinase 1 (pASK-1), phosphorylated c-Jun N-terminal kinase (pJNK), B-cell lymphoma-2 (Bcl-2), and Bcl2-associated X protein (Bax) was examined by immunodetection, and Bcl-2 messenger RNA expression was detected by quantitative polymerase chain reaction. In addition, caspase-3 activity was also measured.

Results

The result of microcirculation analysis showed that the survival and blood perfusion rates significantly increased in the skin flap after HBO exposure. Terminal deoxynucleotidyl transferase dUTP nick-end labeling staining revealed that cell apoptosis was significantly attenuated in the HBO group. Furthermore, HBO preconditioning increased the expression of Bcl-2 and inhibited pASK-1, pJNK, and Bax expression as determined by both immunohistochemistry and Western blot. Caspase-3 activity and the Bax/Bcl-2 ratio declined in the HBO group.

Conclusions

HBO preconditioning effectively ameliorates I/R injury by regulating the apoptosis signal-regulating kinase 1 and/or c-Jun N-terminal kinase pathway and anti- and proapoptosis factors.