Uliginosin B, a natural phloroglucinol derivative with antidepressant-like activity, increases Na⁺,K⁺-ATPase activity in mice cerebral cortex

详细信息    查看全文

• 作者：Ana C. Stein^a ; Liz G. Mü ; ller^a ; André ; a G.K. Ferreira^b ; Andressa Braga^a ; Andresa H. Betti^a ; Fernanda B. Centuriã ; o^a ; Emilene B. Scherer^b ; Janaí ; na Kolling^b ; Gilsane L. von Poser^a ; Angela T.S. Wyse^b ; Stela M.K. Rates^a ; ^{stela.rates@ufrgs.br" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Uliginosin B ; Antidepressant-like activity ; Na+ ; K+-ATPase ; Veratrine ; Voltage-gated sodium channel
• 刊名：Revista Brasileira de Farmacognosia
• 出版年：2016
• 出版时间：September-October 2016
• 年：2016
• 卷：26
• 期：5
• 页码：611-618
• 全文大小：990 K

文摘

Uliginosin B, a phloroglucinol isolated from Hypericum polyanthemum Klotzsch ex Reichardt, Hypericaceae, has antidepressant-like effect in the forced swimming test in rodents and inhibits monoamines neuronal reuptake without binding to their neuronal carriers. Studies showed the involvement of Na⁺,K⁺-ATPase brain activity in depressive disorders, as well as the dependence of neuronal monoamine transport from Na⁺ gradient generated by Na⁺,K⁺-ATPase. This study aimed at evaluating the effect of uliginosin B on Na⁺,K⁺-ATPase activity in mice cerebral cortex and hippocampus (1 and 3 h after the last administration) as well as the influence of veratrine, a Na⁺ channel opener, on the antidepressant-like effect of uliginosin B. Mice were treated (p.o.) with uliginosin B single (10 mg/kg) or repeated doses (10 mg/kg/day, 3 days). Acute administration reduced the immobility in the forced swimming test and tail suspension test and increased Na⁺,K⁺-ATPase activity in cerebral cortex 1 h after treating, whereas the repeated treatment induced the antidepressant-like effect and increased the Na⁺,K⁺-ATPase activity at both times evaluated. None treatment affected the hippocampus enzyme activity. Veratrine pretreatment prevented uliginosin B antidepressant-like effect in the forced swimming test, suggesting the involvement of Na⁺ balance regulation on this effect. Altogether, these data indicate that uliginosin B reduces the monoamine uptake by altering Na⁺ gradient.