Bioinformatic prediction and functional characterization of human KIAA0100 gene

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• 作者：He Cui^a ; ¹ ; Xi Lan^b ; ¹ ; Shemin Lu^b ; Fujun Zhang^b ; Wanggang Zhang^a ; ^{zhangwanggang2003@yahoo.com}
• 关键词：Human KIAA0100 ; Bioinformatic prediction ; Acute monocytic leukemia associated antigen ; CRISPR/Cas9 system ; Cell proliferation ; Cell apoptosis
• 刊名：Journal of Pharmaceutical Analysis
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：7
• 期：1
• 页码：10-18
• 全文大小：1421 K
• 卷排序：7

文摘

Our previous study demonstrated that human KIAA0100 gene was a novel acute monocytic leukemia-associated antigen (MLAA) gene. But the functional characterization of human KIAA0100 gene has remained unknown to date. Here, firstly, bioinformatic prediction of human KIAA0100 gene was carried out using online softwares; Secondly, Human KIAA0100 gene expression was downregulated by the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) 9 system in U937 cells. Cell proliferation and apoptosis were next evaluated in KIAA0100-knockdown U937 cells. The bioinformatic prediction showed that human KIAA0100 gene was located on 17q11.2, and human KIAA0100 protein was located in the secretory pathway. Besides, human KIAA0100 protein contained a signalpeptide, a transmembrane region, three types of secondary structures (alpha helix, extended strand, and random coil) , and four domains from mitochondrial protein 27 (FMP27). The observation on functional characterization of human KIAA0100 gene revealed that its downregulation inhibited cell proliferation, and promoted cell apoptosis in U937 cells. To summarize, these results suggest human KIAA0100 gene possibly comes within mitochondrial genome; moreover, it is a novel anti-apoptotic factor related to carcinogenesis or progression in acute monocytic leukemia, and may be a potential target for immunotherapy against acute monocytic leukemia.