Recognition of a multiple antigen peptide containing sequence from mimotope of the dengue type 3 virus NS4B protein by human antibodies

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• 作者：Nevis Amin¹ ; ^{namin@finlay.edu.cu" class="auth_mail" title="E-mail the corresponding author} ; Maritza Pupo² ; Alicia Aguilar¹ ; Susana Vá ; zquez² ; Yamira Caballero² ; Rolando Ochoa¹ ; Marí ; a G. Guzmá ; n² ; Armando Acosta¹
• 关键词：MAP ; Antibodies ; NS4B ; Dengue virus
• 刊名：Asian Pacific Journal of Tropical Medicine
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：9
• 期：2
• 页码：130-133
• 全文大小：370 K

文摘

To evaluate the recognition of NS4B mimotope, as multiple antigen peptide (MAP), by dengue antibodies presents in serum samples from patients with different serotype infections.

Methods

A MAP containing mimotope sequence was synthesized and used to evaluate the recognition of NS4B mimotope as MAP by a panel of 66 human sera from dengue cases by an indirect ELISA assay.

Results

The MAP differentiated between sera from dengue viruses infected patients and sera from healthy individuals and the best reactivity was shown by serum from dengue type 3 virus patients. The recognition was more intense with serum from patients with secondary infection.

Conclusions

The findings suggest the potential use of NS4B mimotope on the development of a multi-epitope diagnostic tool. These results are important for further immunogenicity studies.