Plasma matrix metalloproteinase 9 as an early surrogate biomarker of advanced colorectal neoplasia

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• 作者：Antonio Z. Gimeno-Garc&iacute ; a^a ; ^{antozeben@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Javier Triñ ; anes^d ; Enrique Quintero^a ; ^c ; Eduardo Salido^b ; ^c ; ^d ; David Nicolá ; s-Pé ; rez^a ; Zaida Adriá ; n-de-Ganzo^a ; Onofre Alarcó ; n-Ferná ; ndez^a ; Beatriz Abrante^a ; Rafael Romero^a ; Marta Carrillo^a ; Laura Ramos^a ; Inmaculada Alonso^a ; Juan Ortega^a ; ^b ; ^c ; ^d ; Alejandro Jimé ; nez^d
• 关键词：Matrix metalloproteinases ; Colorectal cancer ; Advanced adenoma ; Plasma biomarker ; Gelatin zymography
• 刊名：Gastroenterología y Hepatología
• 出版年：2016
• 出版时间：August-September 2016
• 年：2016
• 卷：39
• 期：7
• 页码：433-441
• 全文大小：989 K

文摘

Matrix metalloproteinases (MMPs) are overexpressed at different stages of colorectal carcinogenesis and could serve as early surrogate biomarkers of colorectal neoplasia.

Objective

To assess the utility of plasma MMP2 and MMP9 levels in the detection of advanced colorectal neoplasia and their correlation with tissue levels.

Methods

We analysed blood and tissue samples from patients with non-advanced adenomas (n = 25), advanced adenomas (n = 25), colorectal cancer (n = 25) and healthy controls (n = 75). Plasma and tissue gelatinase levels were determined by Luminex XMAP technology and gelatin zymography. Receiver operating characteristic (ROC) curve analysis was used to calculate the optimum cut-off for the detection of advanced colorectal neoplasia.

Results

Plasma MMP2 levels were similar between groups whatever the type of lesion. Plasma MMP9 levels were significantly higher in patients with neoplastic lesions than in healthy controls (median 292.3 ng/ml vs. 139.08 ng/ml, P < 0.001). MMP9 levels were also higher in colorectal cancer than in non-advanced adenomas (median 314.6 ng/ml vs. 274.3 ng/ml, P = 0.03). There was a significant correlation between plasma and tissue levels of MMP9 (r = 0.5, P < 0.001). The plasma MMP9 cut-off range with the highest diagnostic accuracy was between 173 ng/ml and 204 ng/ml (AUC = 0.80 [95% CI: 0.72–0.86], P < 0.001; sensitivity, 80–86% and specificity, 57–67%).

Conclusion

Plasma MMP9 could be a surrogate biomarker for the early detection of advanced colorectal neoplasia, although its diagnostic performance could be increased by combination with other biomarkers.