A high molecular weight protein Bengalin from the Indian black scorpion (Heterometrus bengalensis C.L. Koch) venom having antiosteoporosis activity in female albino rats
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- 作者：Subhash Haldar ; Shubho Das Gupta ; Aparna Gomes ; Biplab Giri ; Subir Ch ; ra Dasgupta ; Ajay Biswas ; Roshnara Mishra ; Antony Gomes
- 关键词：Osteoporosis ; Experimental osteoporosis ; Scorpion ; Scorpion venom protein ; Bengalin
- 刊名：Toxicon
- 出版年：2010
- 出版时间：February-March 2010
- 年：2010
- 卷：55
- 期：2-3
- 页码：455-461
- 全文大小：232 K
文摘
This study reports the presence of a high molecular weight protein (Bengalin) from the Indian black scorpion (Heterometrus bengalensis) venom having antiosteoporosis activity in experimental osteoporosis developed in female albino Wister rats. Bengalin was purified through DEAE-cellulose ion exchange chromatography and high performance liquid chromatography. The molecular weight of the Bengalin was found to be 72 kDa and the first 20 amino acid sequence was found to be G-P-L-T-I-L-H-I-N-D-V-H-A-A/R-F-E-Q/G-F/G-N-T. Bengalin exhibited significant antiosteoporosis activity in experimental female rats, which was confirmed through analysis of urine Ca2+, PO43−, CRE & OH-P. Bengalin (3 μg and 5 μg/100 g rat/i.p.) antagonized osteoporosis by restoring urinary Ca2+, PO43−, CRE and OH-P, serum/plasma Ca2+, PO43−, ALP, TRAP, PTH, T3, TSH, Osteocalcin, IL1, IL6 and TNF α and bone minerals Ca2+, P, Mg2+, Zn2+, Na+, as compared with the sham operated control rats. Bone minerals density of osteoporosis female rats was improved due to Bengalin, observed through DEXA scan. Subacute toxicity studies in male albino mice, Bengalin showed cardiotoxicity. In vivo experiments, Bengalin showed cardiotoxicity on isolated guinea pig heart, guinea pig auricle, and neurotoxicity on isolated rat phrenic nerve diaphragm preparation. Further detail studies on the toxicity, antiosteoporosis and structural identity of Bengalin are warranted.