In situ label-free quantification of human pluripotent stem cells with electrochemical potential

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• 作者：Cheol-Heon Yea^a ; ^d ; ¹ ; Ho-Chang Jeong^b ; ¹ ; Sung-Hwan Moon^e ; Mi-Ok Lee^f ; Kyeong-Jun Kim^a ; Jeong-Woo Choi^a ; ^c ; ^{jwchoi@sogang.ac.kr" class="auth_mail" title="E-mail the corresponding author} ; Hyuk-Jin Cha^b ; ^{hjcha@sogang.ac.kr" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Human pluripotent stem cells (hPSCs) ; In situ quantification ; Teratoma ; Cyclic voltammetry ; Cell-chip
• 刊名：Biomaterials
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：75
• 期：Complete
• 页码：250-259
• 全文大小：2918 K

文摘

Conventional methods for quantification of undifferentiated pluripotent stem cells such as fluorescence-activated cell sorting and real-time PCR analysis have technical limitations in terms of their sensitivity and recyclability. Herein, we designed a real-time in situ label-free monitoring system on the basis of a specific electrochemical signature of human pluripotent stem cells in vitro. The intensity of the signal of hPSCs highly corresponded to the cell number and remained consistent in a mixed population with differentiated cells. The electrical charge used for monitoring did not markedly affect the proliferation rate or molecular characteristics of differentiated human aortic smooth muscle cells. After YM155 treatment to ablate undifferentiated hPSCs, their specific signal was significantly reduced. This suggests that detection of the specific electrochemical signature of hPSCs would be a valid approach to monitor potential contamination of undifferentiated hPSCs, which can assess the risk of teratoma formation efficiently and economically.