On-column trypsin digestion coupled with LC-MS/MS for quantification of apolipoproteins
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- 作者：Christopher A Toth ; Zsuzsanna Kuklenyik ; ZKuklenyik@cdc.gov" class="auth_mail" title="E-mail the corresponding author ; Jeffrey I Jones ; Bryan A Parks ; Michael S Gardner ; David M Schieltz ; Jon C Rees ; Michael L Andrews ; Lisa G McWilliams ; James L Pirkle ; John R Barr
- 刊名：Journal of Proteomics
- 出版年：2017
- 出版时间：6 January 2017
- 年：2017
- 卷：150
- 期：Complete
- 页码：258-267
- 全文大小：1725 K
文摘
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Simultaneous absolute quantification of 8 apolipoproteins associated with high and low density lipoproteins (HDL and LDL).
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High throughput on-line coupling of immobilized trypsin digestion with quantitative LC-MS/MS analysis.
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Correlations between consensus structures of apolipoproteins and pattern of fast trypsin releasable target peptides.
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Comparison of on-line and off-line batch digestions of apoA-I and apoB-100.
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Multivariate regression analysis used to assess correlations between lipid and apolipoprotein concentrations in patient sera.