Optimization of cytochrome c detection by acoustic and electrochemical methods based on aptamer sensors
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- 作者：Alexandra Poturnayovaa ; bAuthor Vitae ; Gabriela CastillobAuthor Vitae ; Veronika SubjakovabAuthor Vitae ; Marek TatarkobAuthor Vitae ; Maja SnejdarkovaaAuthor Vitae ; Tibor Hianikb ; c ; tibor.hianik@fmph.uniba.sk" class="auth_mail" title="E-mail the corresponding authorAuthor Vitae
- 关键词：DNA aptamer ; Cytochrome c ; Thickness shear mode ; Electrochemical impedance spectroscopy ; Cyclic voltammetry
- 刊名：Sensors & Actuators: B. Chemical
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：238
- 期：Complete
- 页码：817-827
- 全文大小：1512 K
文摘
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Comparative analysis of sensitivity of various DNA aptamers to cytochrome c was performed.
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Cytochrome c was determined with acoustics and impedance aptasensors.
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Best detection limit of aptasensor has been achieved by thickness shear mode acoustic sensor (limit of detection: 0.50 ± 0.05 nM).
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The aptasensor has been validated in blood plasma with good recovery of 92 ± 6.6%.