No correlation exist between sperm hypoosmotic swelling test and sire conception rate (SCR) of Holstein bulls A positive correlation exist between relative sperm volume shift and SCR of Holstein bulls. Sperm aquaporin7 (AQP7) mRNA abundance was positively correlated to both relative volume shift and SCR. AQP7 mRNA expressions and relative sperm volume shift differed (P < 0.05) among low- (<2 SCR), average- (-2 to +2 SCR) and high- (>2 SCR) fertility sire groups. Holstein bulls with higher SCR had significantly greater AQP7 mRNA abundance and contributed to greater regulation of sperm volume shift