Characterization of PIP2 aquaporins in Saccharum hybrids

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• 作者：Larissa Mara de Andrade^a ; ^d ; ^{lari_andrade@hotmail.com" class="auth_mail" title="E-mail the corresponding author} ; Paula Macedo Nobile^a ; ^{paulanobile@yahoo.com" class="auth_mail" title="E-mail the corresponding author} ; Rafael Vasconcelos Ribeiro^e ; ^{rvr@unicamp.br" class="auth_mail" title="E-mail the corresponding author} ; Joã ; o Felipe Nebó ; Carlos de Oliveira^c ; ^{nebo@uol.com.br" class="auth_mail" title="E-mail the corresponding author} ; Antonio Vargas de Oliveira Figueira^c ; ^{figueira@cena.usp.br" class="auth_mail" title="E-mail the corresponding author} ; Luis Tadeu Marques Frigel^a ; ^b ; ^{tadeufrigel@hotmail.com" class="auth_mail" title="E-mail the corresponding author} ; Daniel Nunes^a ; ^{dnunes@iac.sp.gov.br" class="auth_mail" title="E-mail the corresponding author} ; Dilermando Perecin^b ; ^{perecin@fcav.unesp.br" class="auth_mail" title="E-mail the corresponding author} ; Michael dos Santos Brito^a ; ^{msantosbrito@yahoo.com.br" class="auth_mail" title="E-mail the corresponding author} ; Regina Cé ; lia de Matos Pires^f ; ^{rcmpires@iac.sp.gov.br" class="auth_mail" title="E-mail the corresponding author} ; Marcos Guimarã ; es de Andrade Landell^a ; ^{mlandell@iac.sp.gov.br" class="auth_mail" title="E-mail the corresponding author} ; Silvana Creste^a ; ^{screste@iac.sp.gov.br" class="auth_mail" title="E-mail the corresponding author}
• 关键词：AQP ; aquaporin ; Sh ; Sugarcane hybrid ; MIP ; major intrinsic protein ; NCBI ; National Center for Biotechnology Information ; PIP ; plasma membrane intrinsic protein ; qPCR ; quantitative polymerase chain reaction ; SAS ; sugarcane assembled sequences ; SUCEST ; Sugarcane Expressed Sequence Tag database
• 刊名：Plant Gene
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：5
• 期：Complete
• 页码：31-37
• 全文大小：589 K

文摘

Aquaporins (AQPs) are proteins that facilitate the transport of water, small neutral solutes and gases across membranes and have an important role in plant physiology and drought stress responses. The sugarcane (Saccharum spp.) transcriptome was searched for AQPs, also known as major intrinsic proteins. Phylogenetic analysis based on nucleotide sequences identified 33 isoforms that fit into four AQP subfamilies previously described for monocotyledonous: 13 plasma membrane intrinsic protein (PIPs), 11 tonoplast intrinsic proteins (TIPs), six nodulin 26-like intrinsic proteins (NIPs) and three small basic intrinsic proteins (SIPs). Among the PIPs, five proteins were classified as PIP1 type and eight were classified as PIP2 type. The expression profiles of three PIP2 isoforms (ShPIP2;1, ShPIP2;5 and ShPIP2;6), which are counterparts of previously described isoforms involved in drought stress in leaves of higher plants, were aligned with monocots and dicot PIP2 protein sequence showing high identity with maize proteins. Furthermore, the transcript abundance of these three genes was evaluated through quantitative PCR (qPCR) in two sugarcane genotypes (‘IACSP94-2094’ and ‘IACSP97-7065’) subjected to water deficit under field and greenhouse conditions. ShPIP2;1, ShPIP2;5 and ShPIP2;6 isoforms were responsive to water deficit and their expression patterns were dependent on genotype, experimental condition and duration of drought stress. Taken together, our results show that the three APQs have their expression in leaves changed under drought, suggesting that these proteins constitute an important target for functional characterization in sugarcane, particularly focusing the performance of plants under varying water availability.