Integration of cell harvest with affinity-enhanced purification of monoclonal antibodies using aqueous two-phase systems with a dual tag ligand
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- 作者：Isabel Campos-Pintoa ; Edith Espitia-Salomab ; Sara A.S.L. Rosaa ; Marco Rito-Palomaresb ; Oscar Aguilarb ; Miguel Aré ; valo-Rodrí ; guezc ; M. Raquel Aires-Barrosa ; Ana M. Azevedoa ; a.azevedo@tecnico.ulisboa.pt" class="auth_mail" title="E-mail the corresponding author
- 关键词：Aqueous two-phase system ; Cell harvesting ; Affinity partitioning ; Monoclonal antibodies ; Downstream processing
- 刊名：Separation and Purification Technology
- 出版年：2017
- 出版时间：1 February 2017
- 年：2017
- 卷：173
- 期：Complete
- 页码：129-134
- 全文大小：639 K
文摘
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Clarification of animal cell cultures using an affinity aqueous two-phase systems.
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A fused protein named LYTAG-Z was used as affinity ligand for the capture of human antibodies.
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LYTAG shown affinity towards polyethylene glycol – PEG – and co-polymers of ethylene oxide and propylene oxide – EOPO – molecules.
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Antibodies recovered with yield of 89% using a system composed 7% PEG3350 and 6% dextran.