Direct potentiometric quantification of histamine using solid-phase imprinted nanoparticles as recognition elements

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• 作者：Itsaso Basozabal^a ; Antonio Guerreiro^b ; Alberto Gomez-Caballero^a ; M. Aranzazu Goicolea^a ; Ram&oacute ; n J. Barrio^a ; ^{r.barrio@ehu.es" class="auth_mail}
• 关键词：Potentiometric sensor ; Histamine ; Molecularly imprinted polymers ; Solid-phase imprinting ; Nanoparticles
• 刊名：Biosensors and Bioelectronics
• 出版年：15 August, 2014
• 年：2014
• 卷：58
• 期：Complete
• 页码：138-144
• 全文大小：753 K

文摘

A new potentiometric sensor based on molecularly imprinted nanoparticles produced via the solid-phase imprinting method was developed. For histamine quantification, the nanoparticles were incorporated within a membrane, which was then used to fabricate an ion-selective electrode. The use of nanoparticles with high affinity and specificity allowed for label-free detection/quantification of histamine in real samples with short response times. The sensor could selectively quantify histamine in presence of other biogenic amines in real wine and fish matrices. The limit of detection achieved was 1.12脳10^鈭? mol L^鈭?, with a linear range between 10^鈭? and 10^鈭? mol L^鈭? and a response time below 20 s, making the sensor as developed a promising tool for direct quantification of histamine in the food industry.