The Effect of l-Arginine on Dural Healing After Experimentally Induced Dural Defect in a Rat Model

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• 作者：Sayed Ali Ahmadi¹ ; Mostafa Jafari² ; ^{Mostafa.medicine87@gmail.com} ; Mohammad Reza Darabi³ ; Ali Chehrei⁴ ; Masoud Rezaei⁵ ; Marjan Mirsalehi⁶
• 关键词：Arginine ; CSF leakage ; Dura mater ; Dural healing
• 刊名：World Neurosurgery
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：97
• 期：Complete
• 页码：98-103
• 全文大小：1659 K
• 卷排序：97

文摘

Incomplete repair of the dura mater may result in numerous complications such as cerebrospinal fluid leakage and meningitis. For this reason, accurate repair of the dura mater is essential. In this study, the effect of systemic and local supplementation of l-arginine on dural healing was evaluated.MethodsThirty male Wistar rats were used and divided into control, local, and systemic l-arginine groups, with 10 rats in each. In each group, a 5-mm experimental incision was made at the lumbar segment of the dura mater and cerebrospinal fluid leakage was induced. Each group was divided into 2 subgroups and at the end of the first and sixth weeks, the rats were killed and the damaged segments of the dura were separated, histologically evaluated and the dural healing indicators including cell types, granulation tissue formation, collagen deposit, and vascularization were compared between groups.ResultsThe systematic supplementation of l-arginine showed a significant effect in dural healing compared with the control group. After the first week, granulation formation increased considerably (P < 0.031), and after 6 weeks, collagen deposition and neovascularization were significantly different compared with the control group (P < 0.030; P < 0.009). In comparison between different groups at the end of the first and sixth weeks, maximum changes in healing indicators were observed in the systemic group and the least variations were related to the control group.ConclusionsThe systemic supplementation of l-arginine may accelerate dural healing by increasing the level of granulation tissue formation, collagen deposition, and vascularization.