Transcriptional regulation of hypoxia inducible factors alpha (HIF-伪) and their inhibiting factor (FIH-1) of channel catfish (Ictalurus punctatus) under hypoxia

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• 作者：Xin Geng ; Jianbin Feng ; Shikai Liu ; Yaping Wang ; Covadonga Arias ; Zhanjiang Liu
• 关键词：Fish ; Gene ; Hypoxia ; HIF ; FIH-1
• 刊名：Comparative Biochemistry and Physiology, Part B
• 出版年：March, 2014
• 年：2014
• 卷：169
• 期：Complete
• 页码：38-50
• 全文大小：1553 K

文摘

Hypoxia inducible factors (HIFs) are considered to be the master switch of oxygen-dependent gene expression with mammalian species. In most cases, regulation of HIF has been believed at posttranslational levels. However, little is known of HIF regulation in channel catfish, a species highly tolerant to low oxygen condition. Here we report the identification and characterization of HIF-1伪, HIF-2伪a, HIF-2伪b, HIF-3伪, and FIH-1 genes, and their mRNA expression under hypoxia conditions. The transcripts of the five genes were found to be regulated temporally and spatially after low oxygen challenge, suggesting regulation of HIF-伪 genes at pre-translational levels. In most tissues, the HIF-伪 mRNAs were down-regulated 1.5 h but up-regulated 5 h after hypoxia treatment. Of these HIF-伪 mRNAs, the expression of HIF-3伪 mRNA was induced in the most dramatic fashion, both in the speed of induction and the extent of induction, compared to HIF-1伪 and HIF-2伪 genes, suggesting its importance in responses to hypoxia.