Total sesquiterpene lactones prepared from Inula helenium L. has potentials in prevention and therapy of rheumatoid arthritis

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• 作者：Shuang Gao^a ; ¹ ; ^{gaoshuangphu@163.com} ; Qun Wang^a ; ¹ ; ^{qunwang0523@163.com} ; Xin-Hui Tian^b ; ^{tianxinhui@126.com} ; Hui-Liang Li^a ; ^{faranli@hotmail.com} ; Yun-Heng Shen^a ; ^{shenyunheng@hotmail.com} ; Xi-Ke Xu^a ; ^{xkxu@smmu.edu.cn} ; Guo-Zhen Wu^a ; ^{496528260@qq.com} ; Zhen-Lin Hu^a ; ^{zhenlinhu@hotmail.com} ; Wei-Dong Zhang^a ; ^b ; ^{wdzhangy@hotmail.com}
• 关键词：Inula helenium L. ; Total sesquiterpene lactones ; Anti-inflammatory ; Rheumatoid arthritis
• 刊名：Journal of Ethnopharmacology
• 出版年：2017
• 出版时间：20 January 2017
• 年：2017
• 卷：196
• 期：Complete
• 页码：39-46
• 全文大小：1354 K
• 卷排序：196

文摘

Inula helenium L. is an herb with anti-inflammatory properties. Sesquiterpene lactones (SLs), mainly alantolactone (AL) and isoalantolactone (IAL), are considered as its active ingredients. However, the anti-inflammatory effects of SL-containing extracts of I. helenium have not been explored. Here we prepared total SLs from I. helenium (TSL-IHL), analyzed its chemical constituents, and performed cellular and animal studies to evaluate its anti-inflammatory activities.Materials and methodsThe chemical profile of TSL-IHL was analyzed by HPLC-UV. Its in vitro effects on the activation of signaling pathways and expression of inflammatory genes were examined by western blotting and quantitative real-time PCR, respectively, and compared with those of AL and IAL. Its in vivo anti-inflammatory effects were evaluated in adjuvant- and collagen-induced arthritis rat models.ResultsChemical analysis showed that AL and IAL represent major constituents of TSL-IHL. TSL-IHL, as well as AL and IAL, could inhibit TNF-α-induced activation of NF-κB and MAPK pathways in b. End3 cells, suppress the expressions of MMP-3, MCP-1, and IL-1 in TNF-α-stimulated synovial fibroblasts, and IL-1, IL-6, and iNOS in LPS-activated RAW 264.7 cells in a dose-dependent manner in the range of 0.6–2.4 μg/mL. Oral administration of TSL-IHL at 12.5−50 mg/kg could dose-dependently alleviate the arthritic severity and paw swelling in either developing or developed phases of arthritis of rats induced by adjuvant or collagenConclusionsThese results indicated potentials of TSL-IHL in prevention and therapy of rheumatoid arthritis.