Development of a heterologous enzyme-linked immunosorbent assay for the detection of clindamycin and lincomycin residues in edible animal tissues

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• 作者：Jie He^a ; ¹ ; Nan Wu^a ; ¹ ; Pengjie Luo^b ; Ping Guo^c ; Jianwen Qu^a ; Shuying Zhang^a ; Xin Zou^a ; Feiyao Wu^a ; Huijuan Xie^a ; Can Wang^a ; Wenxiao Jiang^a ; ^{jiangwenxiao@szu.edu.cn}
• 关键词：Clindamycin ; Lincomycin ; Antibody ; Immunoassay ; Food safety
• 刊名：Meat Science
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：125
• 期：Complete
• 页码：137-142
• 全文大小：508 K
• 卷排序：125

文摘

In this study, new clindamycin (CLIN) artificial antigens were prepared and used to produce broad-specificity monoclonal antibodies. Based on the as-produced mAbs, a heterologous ELISA was developed to detect CLIN and lincomycin (LIN) residues in edible animal tissues. The IC50 values of the developed assay were 0.3 ng/mL (CLIN) and 1.2 ng/mL (LIN) in buffer, respectively. The detection limits were estimated to be 1.8 μg/kg (CLIN) and 6.8 μg/kg (LIN) in bovine, chicken, porcine and fish muscles. In the spike and recovery tests, the mean recovery rate ranged from 76% to 112% at different spiked levels, and the intra −/inter-assay coefficients of variation were in the range of 7.1% to 13.2%. This method was verified using LC − MS/MS with a correlation coefficient > 0.97. The developed ELISA is therefore well suited for simultaneous determination of CLIN and LIN residues in bovine, chicken, porcine and fish muscles.