Single amino acid substitutions in the hemagglutinin of influenza A/Singapore/21/04 (H3N2) increase virus growth in embryonated chicken eggs

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• 作者：Bin Lu ; Helen Zhou ; Winnie Chan ; George Kemble ; Hong Jin
• 关键词：Influenza A (H3N2) ; Embryonated chicken eggs ; HA receptors ; Reverse genetics
• 刊名：Vaccine
• 出版年：2006
• 出版时间：10 November 2006
• 年：2006
• 卷：24
• 期：44-46
• 页码：6691-6693
• 全文大小：231 K

文摘

Most of the recently circulating H3N2 influenza A strains do not replicate well in embryonated chicken eggs and had to be isolated by cell culture, which presents a great challenge for influenza vaccine production using embryonated chicken eggs. We previously reported that a human H3N2 virus, A/Fujian/411/02, which replicates poorly in eggs, could be improved by changing a minimum of two HA residues (G186V/V226I or H183L/V226A). Here, we extended our work to the A/Singapore/21/04 strain that was also unable to grow in eggs. We showed that a single amino acid substitution of either G186V or A196T in the HA resulted in significantly increased virus replication in eggs without affecting virus antigenicity.