Identification and biochemical characterization of polyamine oxidases in amphioxus: Implications for emergence of vertebrate-specific spermine and acetylpolyamine oxidases
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- 作者：Huihui Wang1 ; Baobao Liu1 ; Hongyan Li ; hongyanli@ouc.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Shicui Zhang ; sczhang@ouc.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：APAO ; N1-acetylpolyamine oxidase ; DNA ; deoxyribonucleic acid ; DTT ; dithiothreitol ; FAD ; flavin adenine dinucleotide ; h ; hour ; HRP ; horseradish peroxidase ; IPTG ; isopropyl β-D-thiogalactoside ; KDa ; kilodalton(s) ; LB ; Luria&ndash ; Bertani (medium) ; M ; mole per liter ; MDL 72 ; 527 ; N1 ; N4-bis (2 ; 3-butadienyl)-1 ; 4-butanediamine ; Min ; minute ; N1-Aspm ; N1-acetylspermine ; N1-Aspd ; N1-acetylspermidine ; ORF ; open reading frame ; PAO ; polyamine oxidase ; PAGE ; PA-gel electrophoresis ; PBS ; phosphate-buffered
- 刊名：Gene
- 出版年：2016
- 出版时间：10 January 2016
- 年：2016
- 卷：575
- 期：2
- 页码：429-437
- 全文大小：1021 K
文摘
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Two polyamine oxidase genes Bjpao1 and Bjpao2 are identified in amphioxus.
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Bjpao1 and Bjpao2 both show distinct tissue-specific expression pattern.
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rBjPAO1 and rBjPAO2 oxidize Spm and N1-Aspm respectively but both oxidase Spd.
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Key residues important for enzymatic activity are identified in BjPAO1 and BjPAO2.
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A model depicting evolution and specialization of SMO and APAO is proposed.