Strong correlation between ASPM gene expression and HCV cirrhosis progression identified by co-expression analysis

详细信息    查看全文

• 作者：Fan Wang^a ; ^b ; Ying Chang^a ; ^b ; Jin Li^a ; ^b ; Hongling Wang^a ; ^b ; Rui Zhou^a ; ^b ; Jian Qi^a ; ^b ; Jing Liu^a ; ^b ; ^{liujing81@hotmail.com} ; Qiu Zhao^a ; ^b ; ^{zhaoqiuwhu@163.com}
• 关键词：ASPM ; Co-expression analysis ; Disease progression ; Hepatitis C virus cirrhosis
• 刊名：Digestive and Liver Disease
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：49
• 期：1
• 页码：70-76
• 全文大小：1756 K
• 卷排序：49

文摘

Hepatitis C virus (HCV) cirrhosis is at a high risk of hepatocellular carcinoma (HCC), and its progression is influenced by a complex network of gene interactions. A weighted gene co-expression network was constructed to identify gene modules associated with the seven-stage disease progression from HCV cirrhosis to HCV-related HCC (n = 65). In the significant module (R2 = 0.86), a total of 25 network hub genes were identified, half of which were also hub nodes in the protein-protein interaction network of the module genes. In validation, most hub genes showed a moderate correlation with the disease progression, and only ASPM was highly correlated (R2 = 0.801). In the test set (n = 63), ASPM was also more highly expressed in HCV cirrhosis with concomitant HCC than in those without HCC (P = 0.0054). Gene set enrichment analysis (GSEA) demonstrated that the gene set of “regulation of protein amino acid phosphorylation” (n = 20) was enriched in HCV cirrhosis samples with ASPM highly expressed (false discovery rate (FDR) = 0.049). In gene ontology (GO) analysis, genes in the enriched set were associated with liver neoplasms and other neoplastic diseases. In conclusion, through co-expression analysis, ASPM was identified and validated in association with the progression of HCV cirrhosis probably by regulating tumor-related phosphorylation.