Suitability of cholinesterase of polychaete Diopatra neapolitana as biomarker of exposure to pesticides: In vitro characterization

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• 作者：Elvira Mennillo^a ; ¹ ; Valentina Casu^a ; ¹ ; Federica Tardelli^a ; ¹ ; Lucia De Marchi^b ; ¹ ; Rosa Freitas^b ; ¹ ; Carlo Pretti^a ; ^c ; ¹ ; ^{carlo.pretti@unipi.it}
• 关键词：Ethylene diamine tetraacetic acid disodium salt ; (EDTA) ; bovine serum albumin ; (BSA) ; CE ; (carboxylesterase) ; ChE ; (cholinesterase) ; AChE ; (acetylcholinesterase) ; BChE ; (butyrylcholinesterase) ; PChE ; (propionylcholinesterase) ; acetylthiocholine iodide ; (ATChI) ; S-butyrylthiocholine iodide ; (BTChI) ; propionylthiocholine iodide ; (PTChI) ; cholinesterase measured by the use of ATChI- ; BTChI- and PTChI as substrate ; (ATChI-ChE ; BTChI-ChE ; PTChI-ChE) ; 1 ; 5-bis 4-allyl dimethyl ammonium phenyl pentan-3-
• 刊名：Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：191
• 期：Complete
• 页码：152-159
• 全文大小：604 K
• 卷排序：191

文摘

Cholinesterases of Diopatra neapolitana were characterized for their activity in whole body and different body segments (apical, intermediate, posterior), substrate affinity (acetyl-, butyryl-, propionylthiocholine), kinetic parameters (Km and Vmax) and in vitro response to model inhibitors (eserine hemisulfate, isoOMPA, BW284C51) and carbamates (carbofuran, methomyl, aldicarb and carbaryl). Results showed that the rate of hydrolysis for acetyl- and propionylthiocholine was higher in the posterior segment than the apical/intermediate segments and whole body. Cholinesterases of D. neapolitana showed a substrate preference for acetylthiocholine followed by propionylthiocholine; butyrylthioline was poorly hydrolyzed indicating, together with the absence of inhibition by the specific inhibitor and the absence of reactive bands in native electrophoresis, a lack of an active butyrylcholinesterase, differently than that observed in other Annelida species. The degree of inhibition by selected carbamates of cholinesterase activity with propionylthiocholine as substrate was higher than that observed with ATChI-ChE activity; aldicarb showed the highest inhibitory effect.