Expression of muscarinic acetylcholine receptors in hepatocytes from rat fibrotic liver

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• 作者：Lin Luo^a ; ^b ; ^{luolin@ntu.edu.cn} ; Tingting Xu^a ; ¹ ; Pengbo Wang^c ; Liuliu Mao^a ; Chenghao Xi^a ; Jinhua Huang^a ; Wei Zhang^a ; ^b ; ^{zwei_601@126.com}
• 关键词：Ach ; acetylcholine ; ALB ; albumin ; ALT ; alanine aminotransferase ; AST ; aspartate aminotransferase ; CCl4 ; carbon tetrachloride ; DMEM ; Dulbecco&rsquo ; s modified Eagle medium ; ECM ; extracellular matrix ; FHCs ; hepatocytes from fibrotic liver ; GSH ; glutathione ; HCs ; hepatocytes ; HF ; hepatic fibrosis ; HSCs ; hepatic stellate cells ; Hyp ; hydroxyproline ; mAchRs ; muscarinic acetylcholine receptors ; MMPs ; matrix metalloproteinases ; Pi ; nitrate pilocarpine ; TIMPs ; tissue inhibitors of metalloproteinases ; RT-
• 刊名：Experimental and Toxicologic Pathology
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：69
• 期：2
• 页码：73-81
• 全文大小：2276 K
• 卷排序：69

文摘

The pathological changes of parasympathetic nerve are considered as an independent prognostic factor of the survival rate of patients with chronic liver disease. The non-selective muscarinic acetylcholine receptors (mAchR) agonists and antagonists can affect the proliferation of hepatocytes and hepatic stellate cells, but the subtypes of mAchR expressions in HCs are still uncertain. Here, we investigate the expression of mAchR in hepatic fibrosis on rats. 3 ml/kg 40% carbon tetrachloride (CCL4) was given to induce hepatic fibrosis on rats and the hepatocytes were isolated. Compared to the normal state, the expression levels of m1, 3, 5 in fibrotic liver tissues or hepatocytes were obviously increased, while m2, 4 decreased. 10 μM pilocarpine or 10 μM acetylcholine could increase the alanine aminotransferase (ALT), hydroxyproline (Hyp), collagen I, III in the hepatocytes, and decreased albumin (ALB). They also changed the expressions of mAchR similarly as the fibrotic hepatocytes and livers. However, atropine could ameliorate the state of fibrotic hepatocytes. These data indicate that mAchR played an important role in the regulation of hepatic fibrosis process. Targeting mAchR would have therapeutic potential for hepatic fibrosis.