In vitro characterization of felid herpesvirus 1 (FHV-1) mutants generated by recombineering in a recombinant BAC vector
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- 作者：S.-H. Sheldon Taia ; b ; Carine Holzb ; Michael D. Engstromc ; Hans H. Chengd ; Roger K. Maesa ; b ; c ; e ; Maes@dcpah.msu.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：ANOVA ; analysis of variance ; BAC ; bacterial artificial chromosome ; BoHV-1 ; bovine herpesvirus 1 ; CRFK cells ; Crandell-Reese feline kidney cells ; EHV-1 ; equid herpesvirus 1 ; FHV-1 ; felid herpesvirus 1 ; gC ; glycoprotein C ; gE ; glycoprotein E ; gI ; glycoprotein I ; HSV-1 ; herpes simplex virus 1 (human herpesvirus 1) ; MOI ; multiplicity of infection ; ORF ; open reading frame ; p.i. ; post infection ; PK ; US3 serine/threonine protein kinase ; PRV ; pseudorabies virus (suid herpesvirus 1) ; TCID50 ; 50% tissue c
- 刊名：Virus Research
- 出版年：2016
- 出版时间：2 August 2016
- 年：2016
- 卷：221
- 期：Complete
- 页码：15-22
- 全文大小：1300 K
文摘
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FHV-1 mutants deficient in gC, PK, gE, or both gE and TK have been generated by BAC clone recombineering.
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PK−, gE−, and gE−TK− mutants produced smaller plaques than the parent strain.
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Except for the gC− mutant, the in vitro growth characteristics of the mutants were similar to those of the parent strain.
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These mutants will be useful for molecular pathogenesis studies and vaccine development.