Green Tea Polyphenols Alleviate Autophagy Inhibition Induced by High Glucose in Endothelial Cells

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• 作者：Pi Wei ZHANG^a ; ^& ; ^{zpwyipdnfk@163.com" class="auth_mail" title="E-mail the corresponding author}Author Vitae ; Chong TIAN^b ; ^& ; ^{tianchong0826@hust.edu.cn" class="auth_mail" title="E-mail the corresponding author}Author Vitae ; Fang Yi XU^a ; Zhuo CHEN^a ; Raynard BURNSIDE^a ; Wei Jie YI^a ; Si Yun XIANG^a ; Xiao XIE^a ; Nan Nan WU^a ; Hui YANG^c ; Na Na ZHAO^d ; Xiao Lei YE^d ; Chen Jiang YING^a ; ^e ; ^{yingcj@hust.edu.cn" class="auth_mail" title="E-mail the corresponding author}
• 刊名：Biomedical and Environmental Sciences
• 出版年：2016
• 出版时间：July 2016
• 年：2016
• 卷：29
• 期：7
• 页码：524-528
• 全文大小：1135 K

文摘

Bovine aortic endothelial cells (BAECs) were cultured with high glucose (33 mmol/L), 4 mg/L green tea polyphenols (GTPs) or 4 mg/L GTPs co-treatment with high glucose for 24 h in the presence or absence of Bafilomycin-A1 (BAF). We observed that high glucose increased the accumulation of LC3-II. Treatment with BAF did not further increase the accumulation of LC3-II. Results also showed an increased level of p62 and decreased Beclin-1. However, GTPs showed inversed trends of those proteins. Furthermore, GTPs co-treatment with high glucose decreased the level of LC3-II and a much higher accumulation of LC3-II was observed in the presence of BAF in comparison with high glucose alone. Results also showed a decreased p62 and increased Beclin-1. The results demonstrated that GTPs alleviated autophagy inhibition induced by high glucose, which may be involved in the endothelial protective effects of green tea against hyperglycemia.