Development of a label-free SPR sensor for detection of matrixmetalloproteinase-9 by antibody immobilization on carboxymethyldextran chip
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- 作者：Sara Mohsenia ; Tahereh Tohidi Moghadama ; Bahareh Dabirmaneshb ; Safoura Jabbarib ; Khosro Khajeha ; b ; khajeh@modares.ac.ir" class="auth_mail" title="E-mail the corresponding author
- 关键词：Surface plasmon resonance ; Matrixmetalloproteinase-9 ; Sensor chip ; Refractive index ; Chronic periodontitis
- 刊名：Biosensors and Bioelectronics
- 出版年：2016
- 出版时间：15 July 2016
- 年：2016
- 卷：81
- 期：Complete
- 页码：510-516
- 全文大小：1460 K
文摘
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Expression, purification and refolding of full length MMP-9 active form in E. coli BL21.
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Immobilization of anti-MMP-9 onto CMD chip surface via amine groups through EDC/NHS.
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Analysis of AFM images showed successful attachment of anti-MMP-9 onto the sensor surface.
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Detection of MMP-9 was designed in the range of 10-200 ng/mL with high sensitivity (LOD 8 pg/mL).
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Performance of SPR immunosensor was tested using saliva sample with average recovery of 94%.