Hydrolase-catalysed synthesis of peroxycarboxylic acids: Biocatalytic promiscuity for practical applications
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- 作者：Chiara Carboni-Oerlemans ; Pablo Domí ; nguez de Marí ; a ; Bernard Tuin ; Gerrald Bargeman ; Ab van der Meer and Robert van Gemert
- 关键词：Biocatalytic promiscuity ; Hydrolases ; Perhydrolysis ; Bleaching ; Epoxidation ; Oxidation
- 刊名：Journal of Biotechnology
- 出版年：2006
- 出版时间：1 November 2006
- 年：2006
- 卷：126
- 期：2
- 页码：140-151
- 全文大小：590 K
文摘
The enzymatic promiscuity concept involves the possibility that one active site of an enzyme can catalyse several different chemical transformations. A rational understanding of the mechanistic reasons for this catalytic performance could lead to new practical applications. The capability of certain hydrolases to perform the perhydrolysis was described more than a decade ago, and recently its molecular basis has been elucidated. Remarkably, a similarity between perhydrolases (cofactor-free haloperoxidases) and serine hydrolases was found, with both groups of enzymes sharing a common catalytic triad, which suggests an evolution from a common ancestor. On the other hand, several biotechnological applications derived from the capability of hydrolases to catalyse the synthesis of peracids have been reported: the use of hydrolases as bleaching agents via in situ generation of peracids; (self)-epoxidation of unsaturated fatty acids, olefins, or plant oils, via Prileshajev epoxidation; Baeyer-Villiger reactions. In the present review, the molecular basis for this promiscuous hydrolase capability, as well as identified applications are reviewed and described in detail.