Regulating effect of activated NF-κB on edema induced by traumatic brain injury of rats

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• 作者：Zi-Ran Wang ; Yu-Xin Li ; Hong-Yan Lei ; Dai-Qun Yang ; Li-Quan Wang ; Ming-Yu Luo ; ^{nyjzlhy@163.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Nuclear transcription factor-κB ; Traumatic brain injury ; Astrocytes ; Rat
• 刊名：Asian Pacific Journal of Tropical Medicine
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：9
• 期：3
• 页码：274-277
• 全文大小：277 K

文摘

To observe the effect of nuclear transcription factor-κB (NF-κB) on cerebral edema in rats with traumatic brain injury (TBI).

Methods

Male SD rats with fluid percussion injury (FPI) were selected. After separation and culture, rats' astrocytes all suffered FPI. The expression of NF-κB and the water content were detected at the animal and cellular levels, while the activity of NOX was evaluated at the cellular level.

Results

According to the results, the positive expression of NF-κB and expression of mRNA were significantly increased and the water content was increased for rats after TBI, while NF-κB inhibitor BAY11-7082 could significantly reduce the effect of TBI. 1 and 3 h after FPI of astrocytes, the activation of NF-κB was increased and BAY 11-7082 could significantly improve the injury-induced swelling of astrocytes. After the injury of astrocytes, the activity of NOX was also increased, while BAY 11-7082 could reduce the activity of NOX.

Conclusions

The results show that the activation of NF-κB in astrocytes is a key factor in the process of cerebral edema after TBI of rats.