How to assess the role of Pt and Zn in the nephrotoxicity of Pt anti-cancer drugs?: An investigation combining μXRF and statistical analysis. Part II: Clinical application

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• 作者：Emmanuel Esteve^a ; Dominique Bazin^c ; ^d ; ^{dominique.bazin@upmc.fr} ; Chantal Jouanneau^a ; Stephan Rouziè ; re^d ; Auré ; lien Bataille^a ; Alex Kellum^c ; Karine Provost^e ; Christian Mocuta^f ; Solenn Reguer^f ; Kris Jorissen^g ; John J. Rehr^g ; Alexandre Hertig^a ; ^h ; Eric Rondeau^a ; ^h ; Emmanuel Letavernier^a ; ^b ; Jean Philippe Haymann^a ; ^b ; Michel Daudon^a ; ^b ; Pierre Ronco^a ; ^b
• 关键词：Nephrotoxicity ; Pt-based anti-cancer drugs X-ray fluorescence ; Synchrotron
• 刊名：Comptes Rendus Chimie
• 出版年：2016
• 出版时间：November-December 2016
• 年：2016
• 卷：19
• 期：11-12
• 页码：1586-1589
• 全文大小：534 K
• 卷排序：19

文摘

In this contribution, an approach developed previously for mice is used for human biopsy. In the case of patient 1, Pt detection is performed 6 days after the last oxaliplatin infusion, while for patient 2, the biopsy was performed more than 15 days after his first platin infusion and several dialysis. Even for these biological samples, experiments show that synchrotron mediated μXRF is a suitable tool to detect Pt in kidney biopsy, and thus probably for any organ exposed to Pt. Therefore, μXRF could also be of major interest to decipher the mechanism beyond Pt induced neurotoxicity, ototoxicity on human biopsy. Pharmacoavailability of chemotherapies is a major concern because some treatment failures are explained by poor tumor penetration of the active molecule. μXRF could be an elegant way to map the distribution of Pt inside cancerous cells at the micrometer scale. Pt and Zn are only two of the numerous trace elements that μXRF can detect; heavy metal intoxication diagnosis and the toxicity mechanism probably could also benefit from this innovative technique.