Scanometric nanomolar lead (II) detection using DNA-functionalized gold nanoparticles and silver stain enhancement

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• 作者：Beibei Chen^aAuthor Vitae ; Zebo Wang^aAuthor Vitae ; Dongxue Hu^aAuthor Vitae ; Qingqing Ma^aAuthor Vitae ; Linna Huang^aAuthor Vitae ; Chengyin Xv^bAuthor Vitae ; Zhiyong Guo^a ; ^{nbuguo@163.com" class="auth_mail} ; ^{guozhiyong@nbu.edu.cn" class="auth_mail}Author Vitae ; Xiaohua Jiang^cAuthor Vitae
• 关键词：Gold label silver stain (GLSS) ; DNAzyme ; Gold nanoparticles ; Lead (Pb2+) detection
• 刊名：Sensors and Actuators B: Chemical
• 出版年：September 2014
• 年：2014
• 卷：200
• 期：Complete
• 页码：310-316
• 全文大小：1396 K

文摘

Based on gold label silver stain method, a facile, sensitive and selective scanometric method for the detection of lead (II) was developed. The main components of the method include “8–17” DNAzyme functionalized gold nanoparticles (AuNP₁), capture DNA functionalized gold nanoparticles (AuNP₂), and silver staining. In presence of lead (II), AuNP₁ was cleaved into different pieces that were captured by AuNP₂; and became aggregated and formed network structures. These aggregate structures hindered the exposure of gold particles during the silver staining, resulting in a reduced gray values compared with that in the absence of lead (II). Using the gray value of silver stain signals, the developed scanometric assay realized a semi-quantitative detection of nanomolar levels of lead (II), ranging from 2 to 1000 nmol/L (about 0.4–200 ppb), without significant interference from other metal ions. Results suggested that this scanometric method is a simple, sensitive, selective, and portable tool for the onsite detection of nanomolar levels of lead (II).