Deleterious Effects of Arsenic, Benomyl and Carbendazim on Human Endometrial Cell Proliferation In Vitro

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• 作者：Chi-Chen Chang^a ; ^b ; ^c ; Yao-Yuan Hsieh^b ; ^† ; ; Kung-Hao Hsu^b ; ^† ; ; Horng-Der Tsai^c ; Wen-Hsin Lin^d ; Chih-Sheng Lin^a ; ^{lincs.biotech@msa.hinet.net} ; ^{lincs@cc.nctu.edu.tw}
• 关键词：arsenic ; benomyl ; carbendazim ; endometrium
• 刊名：Taiwanese Journal of Obstetrics and Gynecology
• 出版年：2010
• 出版时间：December 2010
• 年：2010
• 卷：49
• 期：4
• 页码：449-454
• 全文大小：143 K

文摘

Summary

Objective

We aimed to investigate the effects of arsenic (As), benomyl (Ben), and carbendazim (Carb) on endometrial cells.

Materials and Methods

Human endometrial cells were obtained during diagnostic curettage. All cultured endometrial cells were divided into four groups: (1) 0 M (controls), (2) 10⁻⁶ M, (3) 10⁻⁵ M, (4) 10⁻⁴ M for As, Ben and Carb. After 24 and 48 hours in culture, endometrial cell proliferations were assessed by diphenyltetrazolium bromide assay. The influences of different concentrations of As, Ben and Carb upon the endometrium were compared.

Results

During the first 24 hours, As, Ben and Carb appeared to have insignificant influences upon endometrial growth. After 48 hours in culture, all three agents significantly inhibited endometrial growth. In As groups, cell absorption after 48 hours culture were 100 % (group 1), 82.1 % (group 2), 43.6 % (group 3) and 35.3 % (group 4). In Ben groups, cell absorption was 100 % (1), 75.9 % (2), 66.4 % (3) and 49. 6 % (4). In the Carb groups, cell absorption was 100 % (1), 70.4 % (2), 73.0 % (3) and 76.7 % (4).

Conclusion

The agents As, Ben and Carb appear to have inhibitory effects upon endometrial cells after 48 hours in culture.