Analysis of varicella-zoster virus and herpes simplex virus in various clinical samples by the use of different PCR assays

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• 作者：Niklas Finnströ ; m ; Karin Bergsten ; Helena Strö ; m ; Therese Sundell ; Sophie Martin ; Peter Wutzler ; Andreas Sauerbrei
• 关键词：Herpes simplex virus type 1 ; Herpes simplex virus type 2 ; Varicella-zoster virus ; Polymerase chain reaction ; Virological diagnosis
• 刊名：Journal of Virological Methods
• 出版年：2009
• 出版时间：September 2009
• 年：2009
• 卷：160
• 期：1-2
• 页码：193-196
• 全文大小：299 K

文摘

Rapid and reliable detection of varicella-zoster virus (VZV) and herpes simplex virus type 1 (HSV-1) and -2 (HSV-2) is of clinical significance in immunocompromised patients and patients with infections of the central nervous system. This paper describes the detection of VZV and HSV using the commercially available Affigene^® VZV and Affigene^® HSV 1/2 tracer kits in comparison to “in-house” polymerase chain reaction (PCR) assays. For sample preparation, Qiagen (Hilden, Germany) and Affigene^® (Cepheid AB, Bromma, Sweden) DNA extraction kits were used. 175 samples were analyzed for VZV and 352 samples for HSV-1 and -2. Generally more positive results were obtained using the Affigene^® assays compared to the “in-house” methods independent of the DNA preparation method used. There were significant differences in sensitivity between the Affigene^® HSV 1/2 tracer and the “in-house” PCR assays for the detection of both HSV-1 and -2 in cerebrospinal fluid and vesicle/skin swabs. The Affigene^® HSV 1/2 and VZV tracers are very sensitive assays for detection of VZV and HSV. A wide variety of clinical samples can be examined in combination with either the Qiagen or the Affigene^® DNA extraction kits for preparation.