New azaspiracids in Amphidomataceae (Dinophyceae)
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- 作者：Bernd Krock ; Urban Tillmann ; Daniela Vo? ; Boris P. Koch ; Rafael Salas ; Matthias Witt ; ¨¦ric Potvin ; Hae Jin Jeong
- 关键词：Azaspiracids ; Azadinium poporum ; Amphidoma languida ; LC-MS/MS ; Phycotoxin
- 刊名：Toxicon
- 出版年：2012
- 出版时间：October, 2012
- 年：2012
- 卷：60
- 期：5
- 页码：830-839
- 全文大小：874 K
文摘
Azaspiracids (AZAs) are a group of lipophilic polyether toxins implicated in incidents of shellfish poisoning in humans, particularly in northern Europe, which are produced by the small marine dinoflagellate Azadinium spinosum. Other related species/strains of the Amphidomataceae have not been proven to date to contain any of the known azaspiracids. Closer analyses of these species/strains by triple quadrupole mass spectrometry in the precursor and product ion mode now revealed four new compounds with high similarity to azaspiracids, all of them with a characteristic m/z 348 fragment but with absence of the m/z 362 fragment. These compounds were detected in three species/strains, i.e. in North Sea isolates of Azadinium poporum (molecular mass: 845.5?Da), in a Korean isolate which has been designated as A. cf. poporum (molecular mass: 857.5?Da) and in Amphidoma languida isolated from Bantry Bay, Ireland (molecular masses: 815.5 and 829.5?Da). Cell quotas of roughly 2-20?fg per cell were in the same range as found for AZA-1/-2 in A. spinosum. Structures for all compounds were proposed by interpretation of fragmentation patterns and high resolution mass measurements using Fourier transform ion cyclotron resonance-mass spectrometry (FTICR-MS).