Induction of IL-8(CXCL8) and MCP-1(CCL2) with oxidative stress and its inhibition with N-acetyl cysteine (NAC) in cell culture model using HK-2 cell
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- 作者：Avneesh Kumara ; b ; ; Liliana Shalmanovaa ; Abdul Hammadb ; Stephen E. Christmasc
- 关键词：BCA ; Bicinchoninic acid assay ; DGF ; delayed graft function ; IRI ; Ischaemia reperfusion injury ; GM ; growth media ; GPx ; glutathione peroxidase ; IFN-γ ; Interferon &ndash ; gamma ; IL-1β ; Interleukin 1 beta ; NAC ; N-acetyl cysteine ; NF-κB ; Nuclear factor kappa-light-chain-enhancer of activated B cells ; ROS ; Reactive Oxygen Species ; SFM ; serum free media
- 刊名：Transplant Immunology
- 出版年：2016
- 出版时间：March 2016
- 年：2016
- 卷：35
- 期：Complete
- 页码：40-46
- 全文大小：590 K
文摘
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Oxidative stress is involved in ischaemia-reperfusion injury which also induces chemokines.
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Proximal tubular cell when exposed to hydrogen peroxide induced antioxidant enzyme glutathione peroxidase.
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Hydrogen peroxide also induced chemokines like IL-8 and MCP-1.
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Pre-incubation with anti-oxidant N-acetyl cysteine (NAC) was able to inhibit induction of both chemokines.