Characterization of the porcine peptidylarginine deiminase type VI gene (PADI6) promoter: Sp1 regulates basal transcription of the porcine PADI6
详细信息 查看全文
- 作者：Xiaoliang Xiaa ; xxl8365@163.com" class="auth_mail" title="E-mail the corresponding author ; Chi Yana ; yanchi_2005@126.com" class="auth_mail" title="E-mail the corresponding author ; Wangjun Wub ; wuwangjun2012@njau.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Ying Zhoua ; zhouyingamy@126.com" class="auth_mail" title="E-mail the corresponding author ; Liming Houa ; mnhouliming@126.com" class="auth_mail" title="E-mail the corresponding author ; Bo Zuoa ; zuobo@mail.hzau.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Dequan Xua ; dequanxu@mail.hzau.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Zhuqing Rena ; renzq@mail.hzau.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Yuanzhu Xionga ; xiongyuanzhu@hotmail.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：PAD ; peptidylarginine deiminase ; Sp ; specificity protein ; MZF1 ; myeloid zinc finger 1 ; NF-Y ; nuclear factor-Y ; AP-1 ; activator protein&ndash ; 1 ; GATA ; GATA binding protein ; TSS ; transcription start site ; RACE ; rapid amplification of cDNA ends ; EMSA ; electrophoretic mobility shift assays ; siRNA ; small interfering RNA ; CHO ; Chinese hamster ovary cells ; HeLa ; human cervical carcinoma cells
- 刊名：Gene
- 出版年：2016
- 出版时间：10 January 2016
- 年：2016
- 卷：575
- 期：2
- 页码：551-558
- 全文大小：915 K
文摘
- •
We identified the major transcription start site of the porcine PADI6 gene.
- •
We identified a 153 bp minimal core promoter drove the basal expression of PADI6.
- •
Transcription factor Sp1 bound to the − 56/− 47 region of the minimal promoter.
- •
Overexpression of Sp1 significantly increased the promoter activity of PADI6 gene.
- •
Inhibition of Sp1 significantly reduced the promoter activity of PADI6 gene.