Bioprofiling of unknown antibiotics in herbal extracts: Development of a streamlined direct bioautography using Bacillus subtilis linked to mass spectrometry
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- 作者：Maryam Jamshidi-Aidji Maryam.Jamshidi-Aidji@ernaehrung.uni-giessen.de" class="auth_mail" title="E-mail the corresponding author ; Gertrud E. Morlock ; Gertrud.Morlock@ernaehrung.uni-giessen.de" class="auth_mail" title="E-mail the corresponding author
- 关键词：HPTLC-Bacillus subtilis bioassay ; Bioautography ; Biodensitogram ; Bioautogram ; Antimicrobial compound ; Herbal extract
- 刊名：Journal of Chromatography A
- 出版年：2015
- 出版时间：13 November 2015
- 年：2015
- 卷：1420
- 期：Complete
- 页码：110-118
- 全文大小：2234 K
文摘
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Development of a homogenous plate background for HPTLC-Bacillus subtilis bioassay.
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Introduction of a novel broth for B. subtilis, compared with two common broths.
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Workflow improvement to allow the development of plant extracts with acidic mobile phases.
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Hyphenation with microchemical reactions and mass spectrometry.
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Profiling via HPTLC-Aliivibrio fischeri/尾-glucosidase/acetylcholinesterase assays.