Fast and sensitive detection of foodborne pathogen using electrochemical impedance analysis, urease catalysis and microfluidics
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- 作者：Qi Chena ; Dan Wanga ; Gaozhe Caia ; Yonghua Xiongb ; Yuntao Lic ; Maohua Wangd ; Huiling Huoe ; Jianhan Lina ; jianhan@cau.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Microfluidic impedance biosensor ; Phase angle ; Urease catalysis ; Immunomagnetic separation ; Listeria monocytogenes
- 刊名：Biosensors and Bioelectronics
- 出版年：2016
- 出版时间：15 December 2016
- 年：2016
- 卷：86
- 期：Complete
- 页码：770-776
- 全文大小：1097 K
文摘
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Phase angle shift was successfully used for determination of Listeria.
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The detection limit of this biosensor was 102 CFU/mL for Listeria.
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The detection time was reduced to 1 h due to on-chip passive mixing.
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The recoveries of Listeria cells in the spiked samples was ∼85%.