Conjugation of biotin-coated luminescent quantum dots with single domain antibody-rhizavidin fusions
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- 作者：Jinny L. Liua ; Scott A. Walpera ; Kendrick B. Turnera ; Audrey Brozozog Leeb ; Igor L. Medintza ; Kimihiro Susumuc ; Eunkeu Ohc ; Dan Zabetakisa ; Ellen R. Goldmana ; George P. Andersona ; george.anderson@nrl.navy.mil" class="auth_mail" title="E-mail the corresponding author
- 关键词：Single domain antibodies ; Rhizavidin ; Quantum dots ; Surface plasmon resonance
- 刊名：Biotechnology Reports
- 出版年：2016
- 出版时间：June 2016
- 年：2016
- 卷：10
- 期：Complete
- 页码：56-65
- 全文大小：2994 K
文摘
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Single domain antibody—rhizavidin fusion bioconjugated biotin coated quantum dots.
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Provided facile and effective method to orient antibodies on QD surface.
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Accomplished improved production of His-tagged rhizavidin (RZh) in E. coli.
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Demonstrated utility of RZh as a replacement for tetrameric biotin binders.